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New Sensitive Method for the Measurement of Lysozyme and Lactoferrin to Explore Mucosal Innate Immunity. Part II: Time-Resolved Immunofluorometric Assay Used in HIV Patients with Oral Candidiasis

Abstract : The aim of this study was to explore lysozyme and lactoferrin concentrations in human immunodeficiency virus (HIV)-infected patients with oropharyngeal candidiasis (OPC). These proteins were measured by time-resolved immunofluorometric assay, validated in Part I of this study, in paired serum and salivary secretions of 30 patients. Eleven HIV-positive patients without OPC, eight HIV-positive patients with OPC and eleven HIV-negative healthy subjects were included in the study. The relative coefficient of excretion of salivary albumin was used to establish protein origin. In serum, the low lactoferrin concentrations in HIV-infected patients with and without OPC (0.610 mg/l (p < 0.05) and 0.896 mg/l (p < 0.01) vs. 1.439 mg/l in healthy subjects) were probably due to a decrease in nonspecific immunity, particularly the polymorphonuclear cells. In HIV-infected patients with OPC, the high salivary lysozyme and lactoferrin concentrations (170.94 mg/l and 66.48 mg/l vs. 23.35 mg/l and 10.20 mg/l in healthy subjects, respectively) and their mean relative coefficient of excretion of above 1 indicated a high local production of lysozyme and lactoferrin in saliva. The development of OPC in HIV-infected patients could be a consequence of inefficient lysozyme and lactoferrin concentrations and of decreased cooperation between innate and adaptative immune systems.
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https://hal-univ-fcomte.archives-ouvertes.fr/hal-03534645
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Submitted on : Wednesday, January 19, 2022 - 3:04:39 PM
Last modification on : Wednesday, April 20, 2022 - 1:06:05 PM

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Sophy Laibe, Emmanuel Bard, Sabéha Biichlé, Julien Vielle, Laurence Millon, et al.. New Sensitive Method for the Measurement of Lysozyme and Lactoferrin to Explore Mucosal Innate Immunity. Part II: Time-Resolved Immunofluorometric Assay Used in HIV Patients with Oral Candidiasis. Clinical Chemistry and Laboratory Medicine, De Gruyter, 2003, 41 (2), pp.134-8. ⟨10.1515/CCLM.2003.022⟩. ⟨hal-03534645⟩

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